![]() Kit contents accommodate 32 students (8 4-student workstations) Primary antibody (monoclonal mouse anti-myosin light chain), lyophilized Secondary antibody (polyclonal goat anti-mouse antibody conjugated to myosin peroxidase horseradish (HRP), freeze-dried HRP A color detection reagent HRP B color detection reagent 10x Tris-glycine, 1 L Non-fat dry milk blocker 10x phosphate buffered saline (PBS), 2 x 100 ml Tween 20 10%, 5 ml Nitrocellulose sheets, 0.45 µm (8) Blotting paper sheets (16) Reagent tubes (25) Curriculum including teacher guide, student manual and graphic guide quick. Stain-Free technology adds speed and enables. Serves as a lab extension for the Comparative Proteomics I kit: Protein Profiling Duration Hands-on: Lab activity in four sessions, 45 min per session Bio-Rad Stain-Free western blotting is a streamlined approach to getting better western blotting results. ![]() Quantity: Precision Protein StrepTactin-HRP Conjugate, 300 l. Comprehensive solutions and suggestions are provided to help solve your particular western blotting challenges. ![]() 1.25 ml (5 x 250 l), 10 pre-stained, Strep -tagged recombinant proteins (10250 kD), including three pink reference bands (25, 50, 75 kD), 250 applications. The Western Blot Doctor is a self-help guide developed by Bio-Rad researchers that enables you to identify and troubleshoot western blotting problems. Features and Benefits: Application of Immunology Teaches students to use antibodies as detection tools. Precision Plus Protein WesternC Blotting Standards, 1.25 ml. Proteins were then transferred to a 0,2m nitrocellulose membrane (Trans-Blot Turbo Transfer Pack, Bio-Rad, Cat 1704158) by using a western blot trans-blot. The Immun-Blot AP assay kit includes all necessary reagents for colorimetric detection and can detect up to 100 pg of protein. In this second part of the lab, students apply western blot techniques to their polyacrylamide gel results to specifically identify the myosin light chain among the hundreds of proteins that make up muscle cell extracts from near and distant fish species. Bio-Rad's AP-based colorimetric kits utilize 5-bromo-4-chloro-3-indolyl phosphate/Nitroblue Tetrazolium (BCIP/NBT), which reacts with the alkaline phosphatase to form a color precipitate for detection. ![]() 117179), students use sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) to generate protein profiles and visualize the unique array of proteins that make up the muscle tissues of different fish. In the first part of this lab (Comparative Proteomics Kit I: Protein Profiling, ref. Chemiluminescence and colorimetric detection kits for western blot applications are available in both AP and HRP formats. In A and C, Bio-Rads goat anti-rabbit gold conjugate and gold enhancement kit were used to. The "Comparative Proteomics II: Western Blot Module" kit is the second part of a two-part lab. The membranes were incubated with rabbit anti-human transferrin. ![]()
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